The PDE8A Polyclonal Antibody (CAB12187) is a valuable tool for researchers studying phosphodiesterase 8A (PDE8A), an enzyme involved in the regulation of intracellular levels of cAMP. This antibody, produced in rabbits, specifically targets PDE8A in human samples and is validated for use in Western blotting applications. By binding to PDE8A, this antibody enables researchers to detect and analyze the expression of this enzyme in various cell types, making it ideal for investigations in the fields of cell signaling, metabolic regulation, and neurological disorders.
PDE8A plays a crucial role in modulating cAMP signaling pathways, which are involved in a wide range of physiological processes, including cell growth, hormonal regulation, and neurotransmission. Dysregulation of PDE8A has been implicated in various diseases, such as cancer, cardiovascular disorders, and neurodegenerative conditions. By studying the function of PDE8A using this antibody, researchers can gain insights into the role of cAMP signaling in health and disease, potentially leading to the development of new therapeutic strategies targeting PDE8A activity.
Product Name:
PDE8A Rabbit Polyclonal Antibody
SKU:
CAB12187
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human PDE8A (NP_002596.1).
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5151
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDE8A Rabbit pAb (CAB12187) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
