The PDE6C Polyclonal Antibody (PACO59952) is a valuable tool for researchers studying phosphodiesterase 6C (PDE6C), a key enzyme involved in the regulation of cyclic nucleotide signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PDE6C protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it well-suited for investigations in the fields of molecular biology and pharmacology.PDE6C plays a crucial role in cellular signaling processes by hydrolyzing cyclic nucleotides, which are involved in diverse physiological functions such as vision, smooth muscle relaxation, and neurotransmission.
Dysregulation of PDE6C activity has been implicated in various diseases, including visual disorders and neurological conditions. Consequently, research aimed at elucidating the function and regulatory mechanisms of PDE6C is essential for developing targeted therapies and diagnostic tools for these pathologies.In summary, the PDE6C Polyclonal Antibody (PACO59952) offers researchers a reliable means of investigating the role of PDE6C in signaling pathways and disease processes. Its specificity and sensitivity make it a valuable asset for advancing our understanding of cyclic nucleotide signaling and identifying potential therapeutic targets for a range of medical conditions.
Western Blot. Positive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate. All lanes: PDE6C antibody at 3.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100 kDa. Observed band size: 100 kDa.
Immunofluorescence staining of A549 cells with PACO59952 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PDE6C: Defects in PDE6C are the cause of cone dystrophy type 4 (COD4). An early-onset cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. Belongs to the cyclic nucleotide phosphodiesterase family.Protein type: Phosphodiesterase; EC 3.1.4.35Chromosomal Location of Human Ortholog: 10q24Cellular Component: plasma membraneMolecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding; metal ion bindingBiological Process: metabolic process; phototransduction, visible light; retinal cone cell development; visual perceptionDisease: Cone Dystrophy 4
UniProt Protein Details:
NCBI Summary:
This gene encodes the alpha-prime subunit of cone phosphodiesterase, which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11, 13, and 15 kDa. Mutations in this gene are associated with cone dystrophy type 4 (COD4). [provided by RefSeq, Mar 2010]
