The PDE6C Polyclonal Antibody (PACO19228) is a valuable tool for researchers studying PDE6C, a key enzyme involved in the visual transduction pathway. This antibody, generated in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications. By targeting the PDE6C protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for investigations in ophthalmology and vision science.PDE6C, a phosphodiesterase enzyme found in rod and cone photoreceptor cells, plays a crucial role in the regulation of cyclic nucleotide levels, which are essential for visual signal transduction.
Dysregulation of PDE6C has been implicated in various retinal diseases such as retinitis pigmentosa and congenital stationary night blindness. Therefore, studying the function of PDE6C can provide insights into these vision disorders and potentially lead to the development of targeted therapeutic interventions.
Antibody Name:
PDE6C Antibody (PACO19228)
Antibody SKU:
PACO19228
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human PDE6C
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: A172 cells, Primary antibody: PACO19228(PDE6C Antibody) at dilution 1/1400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
Background:
Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' is an enzyme that in humans is encoded by the PDE6C gene. This gene encodes the alpha-prime subunit of cone phosphodiesterase, which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11, 13, and 15 kDa. Mutations in this gene are associated with cone dystrophy type 4 (COD4).
Synonyms:
phosphodiesterase 6C, cGMP-specific, cone, α prime
UniProt Protein Function:
PDE6C: Defects in PDE6C are the cause of cone dystrophy type 4 (COD4). An early-onset cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. Belongs to the cyclic nucleotide phosphodiesterase family.Protein type: Phosphodiesterase; EC 3.1.4.35Chromosomal Location of Human Ortholog: 10q24Cellular Component: plasma membraneMolecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding; metal ion bindingBiological Process: metabolic process; phototransduction, visible light; retinal cone cell development; visual perceptionDisease: Cone Dystrophy 4
UniProt Protein Details:
NCBI Summary:
This gene encodes the alpha-prime subunit of cone phosphodiesterase, which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11, 13, and 15 kDa. Mutations in this gene are associated with cone dystrophy type 4 (COD4). [provided by RefSeq, Mar 2010]
