The PDE6B Polyclonal Antibody (CAB6942) is a valuable tool for researchers studying the protein Phosphodiesterase 6B (PDE6B), which is involved in the regulation of cyclic nucleotide levels in photoreceptor cells. Raised in rabbits, this antibody is highly specific to human PDE6B samples and is validated for use in Western blot applications. By binding to the PDE6B protein, this antibody enables detection and analysis of PDE6B in various cell types, making it ideal for studies in vision research and retinal diseases.
PDE6B is critical for visual signal transduction in the retina, and mutations in the PDE6B gene are associated with degenerative retinal disorders such as retinitis pigmentosa. Understanding the function and regulation of PDE6B is essential for developing treatments for these conditions and other related diseases. The PDE6B Polyclonal Antibody offers researchers a reliable tool for investigating the role of PDE6B in vision and potential therapeutic strategies.
Product Name:
PDE6B Rabbit Polyclonal Antibody
SKU:
CAB6942
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PDE6B (NP_001138763.1).
Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PDE6B Rabbit pAb (CAB6942) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
