The PDE3B Polyclonal Antibody (CAB8448) is a valuable tool for researchers studying phosphodiesterase 3B (PDE3B), an enzyme involved in the regulation of cyclic adenosine monophosphate (cAMP) levels in cells. This antibody, raised in rabbits, is highly specific to human PDE3B and is validated for use in Western blot applications. By binding to the PDE3B protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for investigations in the fields of metabolism, cardiovascular biology, and cancer research.PDE3B plays a crucial role in cellular signaling pathways that regulate metabolism, cardiac function, and smooth muscle contraction.
Dysregulation of PDE3B expression or activity has been implicated in diseases such as obesity, diabetes, and cardiovascular disorders, making it a promising target for therapeutic interventions. Understanding the function and regulation of PDE3B is key to developing novel treatments for these conditions, and the PDE3B Polyclonal Antibody (CAB8448) provides researchers with a reliable tool for exploring these important biological processes.
Product Name:
PDE3B Rabbit Polyclonal Antibody
SKU:
CAB8448
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human PDE3B (NP_000913.2).
Enables 3',5'-cyclic-nucleotide phosphodiesterase activity. Involved in negative regulation of angiogenesis; negative regulation of cell adhesion; and negative regulation of lipid catabolic process. Located in membrane. Part of guanyl-nucleotide exchange factor complex.
Purification Method:
Affinity purification
Gene ID:
5140
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDE3B antibody (CAB8448) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.
