The PDE1A Polyclonal Antibody (CAB10457) is a valuable tool for researchers studying the phosphodiesterase 1A enzyme, which plays a crucial role in cellular signaling pathways and cAMP regulation. This antibody, produced through rabbit immunization, exhibits strong reactivity with human samples and has been validated for use in Western blot applications.PDE1A is known to be involved in various physiological processes, including smooth muscle contraction, cardiac function, and neuronal signaling. Dysregulation of PDE1A has been linked to conditions such as heart failure, hypertension, and neurological disorders.
By targeting PDE1A with this specific antibody, researchers can investigate its expression levels and activity in different cell types and tissues.The ability to detect and analyze PDE1A protein expression using this antibody makes it an essential tool for studies in pharmacology, neuroscience, and cardiovascular research. Understanding the role of PDE1A in cellular signaling pathways is key to developing novel therapies for treating diseases associated with its dysregulation.
Product Name:
PDE1A Rabbit Polyclonal Antibody
SKU:
CAB10457
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 446-545 of human PDE1A (NP_005010.2).
Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).
Purification Method:
Affinity purification
Gene ID:
5136
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PDE1A antibody (CAB10457) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
