The PDCL3 Polyclonal Antibody (PAC063891) is a powerful tool for researchers investigating PDCL3, a protein involved in cell cycle regulation and DNA repair processes. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the PDCL3 protein, it enables accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and cancer research.PDCL3, also known as plasmid DNA-binding protein 1, plays a crucial role in maintaining genomic stability and ensuring proper cell division.
Its involvement in DNA damage response pathways and cell cycle control makes it a promising target for studying diseases such as cancer, where disruptions in these processes can lead to uncontrolled cell growth and tumor formation. Understanding the function of PDCL3 is key to uncovering new therapeutic strategies for treating cancer and other related disorders.
Western Blot. Positive WB detected in: A2780 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: PDCL3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa. Observed band size: 37 kDa.
Immunofluorescence staining of HepG2 cells with PACO63891 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Acts as a chaperone for the angiogenic VEGF receptor KDR/VEGFR2, controlling its abundance and inhibiting its ubiquitination and degradation. Modulates the activation of caspases during apoptosis. Is a substrate for Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) IAP-mediated ubiquitination.
PDCL3: Modulates the activation of caspases during apoptosis. Is a substrate for Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) IAP-mediated ubiquitination. Belongs to the phosducin family.Protein type: ApoptosisChromosomal Location of Human Ortholog: 2q11.2Cellular Component: cytoplasm; nucleusMolecular Function: protein binding; queuine tRNA-ribosyltransferase activityBiological Process: positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; queuosine biosynthetic process; regulation of peptidyl-tyrosine phosphorylation
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the phosducin-like protein family and is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin. Members of the phosducin-like protein family have been shown to bind to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008]
