The PDCD6IP Polyclonal Antibody (CAB2215) is a valuable tool for researchers studying PDCD6IP, a protein known for its role in apoptosis and intracellular calcium homeostasis. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to PDCD6IP, researchers can effectively detect and analyze this protein in various cell types, facilitating studies in cell biology, apoptosis, and calcium signaling pathways.PDCD6IP, also called Programmed cell death 6 Interacting Protein, is involved in various cellular processes, including cell death, cell cycle regulation, and intracellular signaling.
Understanding the function and regulation of PDCD6IP is crucial for unraveling its implications in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. The PDCD6IP Polyclonal Antibody provides researchers with a reliable tool to investigate the role of PDCD6IP in these pathological conditions and potentially develop targeted therapies.
Product Name:
ALIX/PDCD6IP Rabbit Polyclonal Antibody
SKU:
CAB2215
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALIX / PDCD6IP (NP_037506.2).
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
Purification Method:
Affinity purification
Gene ID:
10015
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALIX / PDCD6IP antibody (CAB2215) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
