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PDCD4 (Phospho-Ser67) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB01474
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Death
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
$719
Frequently bought together:

Description

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PDCD4 (Phospho-Ser67)Colorimetric Cell-Based ELISA Kit

The PDCD4 Phospho-Ser67 Colorimetric Cell-Based ELISA Kit is a cutting-edge assay designed for the accurate and sensitive detection of phosphorylated PDCD4 at serine 67 in cell lysates. This kit is optimized for use in cell-based experiments and provides high specificity and reproducibility, ensuring reliable results every time. Phosphorylation of PDCD4 at serine 67 is a key regulatory mechanism that modulates its activity and function in various cellular processes, including cell growth, apoptosis, and metastasis.

Dysregulation of PDCD4 phosphorylation has been implicated in cancer progression and drug resistance, highlighting its importance as a potential therapeutic target. With this PDCD4 Phospho-Ser67 Colorimetric Cell-Based ELISA Kit, researchers can delve deeper into the molecular mechanisms underlying cancer development and progression, opening new avenues for the development of targeted therapies and personalized medicine approaches.

Product Name:PDCD4 (Phospho-Ser67) Colorimetric Cell-Based ELISA
Product Code:CBCAB01474
ELISA Type:Cell-Based
Target:PDCD4 (Phospho-Ser67)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The PDCD4 (Phospho-Ser67) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PDCD4 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated PDCD4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDCD4 phosphorylation.

Qualitative determination of PDCD4 (Phospho-Ser67) concentration is achieved by an indirect ELISA format. In essence, PDCD4 (Phospho-Ser67) is captured by PDCD4 (Phospho-Ser67)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 27250, UniProt ID: Q53EL6, OMIM: 608610, Unigene: Hs.711490
Gene Symbol:PDCD4
Sub Type:Phospho
UniProt Protein Function:PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.
UniProt Protein Details:

Protein type:Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:protein binding; RNA binding

Biological Process: apoptosis; cell aging; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of JNK activity; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
UniProt Code:Q53EL6
NCBI GenInfo Identifier:317373317
NCBI Gene ID:27250
NCBI Accession:Q53EL6.2
UniProt Secondary Accession:Q53EL6,O15501, Q5VZS6, Q6PJI5, Q8TAR5, Q99834, B2RCV4 B5ME91,
UniProt Related Accession:Q53EL6
Molecular Weight:50,576 Da
NCBI Full Name:Programmed cell death protein 4
NCBI Synonym Full Names:programmed cell death 4 (neoplastic transformation inhibitor)
NCBI Official Symbol:PDCD4  
NCBI Official Synonym Symbols:H731  
NCBI Protein Information:programmed cell death protein 4
UniProt Protein Name:Programmed cell death protein 4
UniProt Synonym Protein Names:Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
Protein Family:Programmed cell death protein
UniProt Gene Name:PDCD4  
UniProt Entry Name:PDCD4_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-PDCD4 (Phospho-Ser67) Antibody, Anti-PDCD4 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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