The CD274 Polyclonal Antibody (CAB11273) is a powerful tool for researchers studying the CD274 protein, commonly known as PD-L1, a key player in immune checkpoint pathways. PD-L1 is known to suppress the immune response, making it a critical target for cancer immunotherapy research. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.By specifically binding to the CD274 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for studies in cancer biology and immunology.
Understanding the role of PD-L1 in immune evasion and tumor immune escape is essential for developing effective cancer therapies. The CD274 Polyclonal Antibody is a valuable tool for investigating the mechanisms underlying PD-L1-mediated immunosuppression and for exploring novel treatments that target this pathway.
Product Name:
PD-L1/CD274 Rabbit Polyclonal Antibody
SKU:
CAB11273
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 19-238 of human PD-L1/CD274 (NP_054862.1).
Cell membrane, Endomembrane system, Single-pass type I membrane protein.
Calculated MW:
33kDa
Observed MW:
40-50kDa
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
29126
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PD-L1/CD274 Rabbit pAb (CAB11273) at 1:500 dilution.A-549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
![FITC Anti-Human CD274/PD-L1 Antibody [29E.2A3] (AGEL1648)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22179/607300/fitc-anti-human-cd274pd-l1-antibody-29e.2a3-agel1648__30241.1707500711.jpg?c=2 "FITC Anti-Human CD274/PD-L1 Antibody [29E.2A3] (AGEL1648)")
![Biotin Anti-Mouse CD274/PD-L1 Antibody [10F.9G2] (AGEL1638)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22169/607052/biotin-anti-mouse-cd274pd-l1-antibody-10f.9g2-agel1638__07218.1707499979.jpg?c=2 "Biotin Anti-Mouse CD274/PD-L1 Antibody [10F.9G2] (AGEL1638)")
![FITC Anti-Mouse CD274/PD-L1 Antibody [10F.9G2] (AGEL2741)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/229366/605234/fitc-anti-mouse-cd274pd-l1-antibody-10f.9g2-agel2741__65790.1706889767.jpg?c=2 "FITC Anti-Mouse CD274/PD-L1 Antibody [10F.9G2] (AGEL2741)")
![Biotin Anti-Human CD274/PD-L1 Antibody [29E.2A3] (AGEL1647)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22178/605476/biotin-anti-human-cd274pd-l1-antibody-29e.2a3-agel1647__80050.1707494958.jpg?c=2 "Biotin Anti-Human CD274/PD-L1 Antibody [29E.2A3] (AGEL1647)")