The PCSK9 Polyclonal Antibody (CAB7860) is a crucial tool for researchers studying PCSK9, a protein involved in regulating cholesterol levels in the body. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in various applications including Western blot analysis.PCSK9, a secreted protein primarily expressed in the liver, plays a key role in regulating the levels of low-density lipoprotein (LDL) cholesterol in the blood by promoting the degradation of the LDL receptor. Dysregulation of PCSK9 has been linked to familial hypercholesterolemia and cardiovascular diseases, making it a promising target for therapeutic interventions.
By using the PCSK9 Polyclonal Antibody, researchers can effectively detect and analyze PCSK9 protein expression in different cell types and tissues, providing valuable insights into its function and potential implications for cardiovascular health. This antibody is a valuable tool for studies focusing on lipid metabolism, atherosclerosis, and other related conditions, ultimately contributing to the development of novel therapeutic strategies for managing cholesterol levels and reducing cardiovascular risk.
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
255738
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Proprotein Convertase 9(PCSK9) Rabbit pAb (CAB7860) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
