The PCSK7 Polyclonal Antibody (PAC057916) is specifically designed for research involving PCSK7, a key enzyme involved in the processing of proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PCSK7 protein, this antibody allows for the detection and analysis of PCSK7 in various cell types, making it a valuable tool for studies in protein processing and related research areas.PCSK7, also known as Proprotein Convertase Subtilisin/Kexin Type 7, plays a crucial role in proteolytic processing of protein precursors and regulation of biological processes.
Its involvement in protein maturation and activation makes it a promising target for research into diseases such as cancer, neurodegenerative disorders, and metabolic conditions. Understanding the function of PCSK7 is essential for developing potential therapies that target protein processing pathways and modulate disease progression.
Antibody Name:
PCSK7 Antibody (PACO57916)
Antibody SKU:
PACO57916
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Proprotein convertase subtilisin/kexin type 7 protein (189-362AA)
IHC image of PACO57916 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Serine endoprotease that processes various proproteins by cleavage at paired basic amino acid, , recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive secretory pathway.
PCSK7: Likely to represent a ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif. Belongs to the peptidase S8 family.Protein type: Protease; Membrane protein, integral; EC 3.4.21.-Chromosomal Location of Human Ortholog: 11q23-q24Cellular Component: integral to Golgi membraneMolecular Function: peptidase activity; serine-type endopeptidase activityBiological Process: protein processing
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes and plays a role in the regulation of iron metabolism. A t(11;14)(q23;q32) chromosome translocation associated with B-cell lymphoma occurs between this gene and its inverted counterpart. [provided by RefSeq, Feb 2014]
