The PCSK1 Polyclonal Antibody (CAB12237) is a valuable tool for researchers studying PCSK1, a proprotein convertase enzyme involved in the processing of precursor proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the PCSK1 protein, this antibody enables accurate detection and analysis in various cell types, making it essential for studies in molecular biology and endocrinology.PCSK1 plays a crucial role in the post-translational processing of prohormones and proneuropeptides, leading to the production of biologically active peptides.
Dysregulation of PCSK1 activity has been implicated in various metabolic disorders, making it a promising target for therapeutic interventions. By exploring the functions of PCSK1, researchers can gain insights into the mechanisms underlying obesity, diabetes, and other metabolic diseases, paving the way for the development of novel treatment strategies.
Product Name:
PCSK1 Rabbit Polyclonal Antibody
SKU:
CAB12237
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-170 of human PCSK1 (NP_000430.3).
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene
Purification Method:
Affinity purification
Gene ID:
5122
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of rat brain, using PCSK1 antibody (CAB12237) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
