The PCNA Rabbit Monoclonal Antibody offered by Assaygenie (product code: AM006) is a valuable tool for researchers studying proliferating cell nuclear antigen (PCNA), a protein essential for DNA replication and repair processes. This antibody, developed using rabbit monoclonal technology, is highly specific and sensitive for detecting PCNA in a variety of samples, making it suitable for applications such as immunohistochemistry and immunofluorescence.PCNA is a marker of cell proliferation and is often upregulated in cancer cells, making it a target of interest for cancer research. By using the PCNA Rabbit Monoclonal Antibody, researchers can easily visualize and quantify PCNA levels in cells and tissues, providing valuable insights into cell cycle progression and DNA repair mechanisms.
Furthermore, the PCNA Rabbit Monoclonal Antibody has been extensively validated and optimized for use in various experimental conditions, ensuring reliable and reproducible results. Whether investigating cell growth, DNA replication, or cellular response to genotoxic stress, this antibody is a vital tool for advancing research in fields such as oncology, cell biology, and molecular genetics.In summary, the PCNA Rabbit Monoclonal Antibody from Assaygenie offers researchers a high-quality reagent for studying PCNA and its role in cellular processes, providing valuable data that can contribute to a better understanding of cancer biology and potential therapeutic targets.
Product Name:
PCNA Rabbit Monoclonal Antibody
SKU:
CAB12427
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,African green monkey
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 162-261 of human PCNA (NP_002583.1).
HeLa cells,MCF7 cells,293T cells,C2C12 cells,COS-7 cells,Mouse spleen,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus
Calculated MW:
29kDa
Observed MW:
35kDa
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Purification Method:
Affinity purification
Gene ID:
5111
Clone Number:
ARC51324
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PCNA Rabbit mAb (CAB12427) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
