The PCNA Polyclonal Antibody (CAB13336) is a valuable tool for researchers studying proliferating cell nuclear antigen (PCNA), a protein involved in DNA replication and repair processes. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it a reliable option for Western blot applications.PCNA is a key player in maintaining genome stability and is essential for cell proliferation. Dysregulation of PCNA expression or function has been linked to various diseases, including cancer. By targeting PCNA with this antibody, researchers can analyze the protein's levels and localization in different cell types, providing insights into its role in cell cycle regulation and DNA damage response mechanisms.
Furthermore, the PCNA Polyclonal Antibody is suitable for studies in fields such as oncology, molecular biology, and genetics, where understanding PCNA function is crucial for developing targeted therapies and diagnostics. With its specificity and sensitivity, this antibody is a valuable asset for advancing research into the roles of PCNA in health and disease.
Product Name:
PCNA Rabbit Polyclonal Antibody
SKU:
CAB13336
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 162-261 of human PCNA (NP_002583.1).
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Purification Method:
Affinity purification
Gene ID:
5111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PCNA Rabbit pAb (CAB13336) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
