The PCNA Polyclonal Antibody (CAB0264) is a valuable tool for research involving Proliferating Cell Nuclear Antigen (PCNA), a protein involved in DNA replication and repair processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PCNA protein, researchers can accurately detect and analyze its expression in various cell types, making it an ideal choice for studies in molecular biology, cancer research, and genetics.PCNA is known for its essential role in DNA synthesis and cell proliferation, making it a crucial factor in maintaining genomic stability and supporting tumor growth in cancer cells.
Understanding the expression and activity of PCNA is vital for unraveling the mechanisms underlying cell division and DNA repair processes, with implications for developing targeted therapies for cancer treatment and other genetic disorders.Overall, the PCNA Polyclonal Antibody (CAB0264) is a reliable tool for investigating the functions of PCNA in cellular processes, offering researchers the opportunity to delve deeper into the molecular mechanisms that drive genetic replication and repair pathways.
Product Name:
PCNA Rabbit Polyclonal Antibody
SKU:
CAB0264
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 162-261 of human PCNA (NP_002583.1).
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Purification Method:
Affinity purification
Gene ID:
5111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PCNA antibody (CAB0264) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
