The PCK2 Polyclonal Antibody (CAB8446) is a valuable tool for research focused on PCK2, a key enzyme involved in the regulation of glucose metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By binding specifically to the PCK2 protein, this antibody enables reliable detection and analysis in a variety of cell types, making it an excellent choice for studies in metabolic regulation, cancer metabolism, and metabolic diseases.PCK2, also known as phosphoenolpyruvate carboxykinase 2, plays a crucial role in gluconeogenesis and glyceroneogenesis, processes essential for maintaining glucose homeostasis and energy balance in the body.
Dysregulation of PCK2 activity has been implicated in diseases such as cancer, diabetes, and metabolic disorders, highlighting its importance as a potential therapeutic target. By elucidating the function of PCK2, researchers can gain valuable insights into metabolic pathways and develop novel strategies for treating metabolic disorders and cancer.
Product Name:
PEPCK/PCK2 Rabbit Polyclonal Antibody
SKU:
CAB8446
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5106
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells using PEPCK/PCK2 Rabbit pAb(CAB8446) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
