The PEPCK/PCK2 Polyclonal Antibody is a valuable tool for researchers investigating the role of the PEPCK and PCK2 proteins in cellular metabolism and energy production. This antibody is raised in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of PEPCK and PCK2 in a variety of cell types.PEPCK and PCK2 are key enzymes involved in gluconeogenesis, the process by which glucose is synthesized from non-carbohydrate sources. These enzymes play a crucial role in regulating blood sugar levels and are therefore of interest in studies of diabetes, obesity, and metabolic disorders.
By targeting PEPCK and PCK2, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the PEPCK/PCK2 Polyclonal Antibody offers researchers a reliable tool for investigating the function and regulation of PEPCK and PCK2 in cellular metabolism, with potential implications for the development of novel treatments for metabolic diseases.
Product Name:
PEPCK/PCK2 Polyclonal Antibody
SKU:
CAB21529
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
PEPCK; PEPCK2; PEPCK-M; K2
Positive Sample:
293T,SH-SY5Y,HepG2
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
71kDa
Observed MW:
71kDa
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
5106
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] PEPCK/PCK2 Rabbit pAb (CAB21529) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
