The PCK1 Monoclonal Antibody is a valuable tool for researchers studying the protein phosphoenolpyruvate carboxykinase 1 (PCK1), which plays a crucial role in gluconeogenesis and is a key enzyme in glucose metabolism. This antibody, produced by Assay Genie, is specifically designed to target and identify PCK1 in various cell types and tissues, making it an ideal choice for studies in metabolic pathways, diabetes, and liver function.This monoclonal antibody, developed using hybridoma technology, is highly specific and sensitive, ensuring accurate and reliable results in experiments such as Western blotting and immunohistochemistry.
Its reactivity with human samples has been extensively validated, guaranteeing consistent performance in research settings.With the ability to detect and quantify PCK1 expression, researchers can gain valuable insights into the regulation of glucose production and utilization in health and disease. The PCK1 Monoclonal Antibody is a powerful tool for advancing our understanding of metabolic disorders and developing targeted therapeutic strategies for conditions related to glucose metabolism dysregulation.
Product Name:
PCK1 Monoclonal Antibody
SKU:
CAB22172
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 523-622 of human PCK1 (NP_002582.3).
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
Purification Method:
Affinity purification
Gene ID:
5105
Clone Number:
ARC56074
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PCK1 antibody (CAB22172) at1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
