The PCGF6 Polyclonal Antibody (CAB5760) is a valuable tool for researchers studying PCGF6, a protein involved in gene regulation and chromatin organization. This antibody, generated from rabbit hosts, exhibits high reactivity with human samples and is optimized for Western blot applications. By specifically binding to the PCGF6 protein, this antibody facilitates the detection and analysis of PCGF6 expression in various cell types, offering insight into its role in cellular processes.PCGF6, a component of the polycomb repressive complex 1 (PRC1), plays a crucial role in gene silencing and transcriptional regulation. Its involvement in chromatin dynamics and gene expression makes it a key player in cellular development and differentiation.
Research on PCGF6 is pertinent to understanding epigenetic regulation, stem cell maintenance, and cancer progression, offering potential therapeutic targets for diseases related to dysregulated gene expression.The PCGF6 Polyclonal Antibody is a reliable tool for investigating the function and regulation of PCGF6 in biological systems. By providing a means to detect and quantify PCGF6 expression levels, this antibody aids in unraveling the molecular mechanisms underlying chromatin modification and gene transcription, paving the way for advancements in the fields of epigenetics, developmental biology, and cancer research.
Product Name:
PCGF6 Rabbit Polyclonal Antibody
SKU:
CAB5760
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 161-350 of human PCGF6 (NP_001011663.1).
The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
84108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of MCF7 cells, using PCGF6 antibody (CAB5760) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
