The PCDHA13 Polyclonal Antibody (CAB7475) is a valuable tool for researchers studying PCDHA13, a cell adhesion molecule that plays a role in neuronal development and synaptic transmission. This antibody, produced in rabbits, shows high specificity and sensitivity for PCDHA13 in human samples, making it ideal for Western blot applications. By binding to the PCDHA13 protein, this antibody enables researchers to detect and analyze PCDHA13 expression in various cell types, providing insights into its function in neurobiology and developmental biology.
PCDHA13 is a member of the protocadherin family, which is involved in cell-cell adhesion and communication in the nervous system. The unique expression and functions of PCDHA13 make it a fascinating target for research into neurological disorders, such as autism and schizophrenia, as well as developmental abnormalities. Investigating the role of PCDHA13 with the help of this antibody may lead to a better understanding of neuronal connectivity and potential therapeutic targets for neurological conditions.
Product Name:
PCDHA13 Rabbit Polyclonal Antibody
SKU:
CAB7475
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-360 of human PCDHA13 (NP_061727.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
102kDa
Observed MW:
90kDa
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.
Purification Method:
Affinity purification
Gene ID:
56136
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PCDHA13 antibody (CAB7475) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
