The PCDHA12 Polyclonal Antibody (CAB8501) is a valuable tool for researchers studying PCDHA12, a member of the protocadherin alpha gene cluster that plays a role in neuronal development and synaptic connectivity. This antibody, produced in rabbits, has high specificity and sensitivity for detecting PCDHA12 in human samples, making it useful for various research applications such as immunofluorescence and immunohistochemistry.PCDHA12 is essential for neuronal circuit formation and synaptic plasticity, making it a crucial molecule in understanding brain development and function.
Dysregulation of PCDHA12 has been implicated in neurological disorders such as autism and schizophrenia, making it a key target for further investigation in these areas. By studying the expression and function of PCDHA12 using this antibody, researchers can gain valuable insights into the mechanisms underlying these complex disorders and potentially identify new therapeutic targets.
Product Name:
PCDHA12 Rabbit Polyclonal Antibody
SKU:
CAB8501
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 713-792 of human PCDHA12 (NP_114070.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
102kDa
Observed MW:
102kDa
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.
Purification Method:
Affinity purification
Gene ID:
56137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PCDHA12 antibody (CAB8501) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
