The PCCB Polyclonal Antibody (CAB5415) is a valuable tool for researchers studying the PCCB protein, which is involved in the breakdown of valine, isoleucine, and methionine. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the PCCB protein, researchers can easily detect and analyze its expression in various cell types, making it an essential tool for studies in metabolic disorders, enzyme deficiencies, and other related research areas.The PCCB protein plays a critical role in maintaining metabolic homeostasis and energy production by catalyzing the final step in the breakdown of certain amino acids.
Dysregulation of PCCB activity can lead to severe disorders, such as propionic acidemia, which highlights the importance of studying this protein for potential therapeutic interventions. By gaining a deeper understanding of PCCB function, researchers can pave the way for developing targeted treatments and interventions for individuals affected by metabolic disorders.
Product Name:
PCCB Rabbit Polyclonal Antibody
SKU:
CAB5415
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-539 of human PCCB (NP_000523.2).
The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5096
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PCCB antibody (CAB5415) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
