The PCCA Polyclonal Antibody (CAB9486) is a valuable tool for researchers studying the PCCA gene, which codes for the protein propionyl-CoA carboxylase alpha chain. This enzyme plays a key role in the breakdown of certain amino acids and fatty acids in the body. Raised in rabbits, this antibody is highly specific to human samples and is validated for use in Western blot applications. It binds specifically to the PCCA protein, allowing for accurate detection and analysis in a variety of cell types. Research involving the PCCA gene and its protein product is essential in understanding metabolic diseases such as propionic acidemia, a rare inherited disorder characterized by the body's inability to break down certain amino acids and fats.
By studying the function of PCCA, researchers can develop potential therapeutic strategies for treating individuals affected by these conditions. Overall, the PCCA Polyclonal Antibody is a reliable tool for scientists conducting research in the fields of metabolism, genetics, and rare diseases. Its high reactivity and specificity make it an essential component in investigations aimed at unraveling the complexities of PCCA-related disorders.
Product Name:
PCCA Rabbit Polyclonal Antibody
SKU:
CAB9486
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 449-728 of human PCCA (NP_000273.2).
The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5095
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PCCA Rabbit pAb (CAB9486) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
