The PCB2 Polyclonal Antibody (CAB2531) is a valuable tool for researchers studying PCB2, a protein involved in RNA binding and regulation. This antibody, generated in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the PCB2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studying RNA metabolism, gene expression, and post-transcriptional regulation.PCB2 is known for its role in modulating RNA stability and translation efficiency, making it a key player in gene expression regulation. Its functions are crucial for cellular processes such as mRNA localization, degradation, and protein synthesis control.
Given its involvement in RNA metabolism, PCB2 is a promising target for research in areas such as molecular biology, developmental biology, and neurobiology.By using the PCB2 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying RNA regulation and gene expression control. This antibody is an essential tool for investigating the functions of PCB2 and its impact on various cellular processes, making it a valuable asset for studies focused on RNA biology and related fields.
Product Name:
PCBP2 Rabbit Polyclonal Antibody
SKU:
CAB2531
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human hnRNP E2/PCBP2 (NP_001122383.1).
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5094
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using hnRNP E2/PCBP2 Rabbit pAb (CAB2531) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
