The PCBP1 Polyclonal Antibody (CAB1044) is a valuable tool for researchers studying PCBP1, a protein involved in various cellular processes such as RNA processing and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the PCBP1 protein, enabling accurate detection and analysis in a variety of cell types.PCBP1, also known as Poly(rC)-binding protein 1, plays a critical role in post-transcriptional regulation of gene expression by binding to target mRNAs.
Its involvement in RNA metabolism makes it a key player in the control of cellular functions and has implications in disease processes such as cancer and viral infections. Understanding the functions and interactions of PCBP1 is essential for unraveling its role in cellular biology and developing potential therapeutic strategies targeting this protein.
Product Name:
hnRNP E1/PCBP1 Rabbit Polyclonal Antibody
SKU:
CAB1044
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/hnRNP E1/PCBP1 (NP_006187.2).
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Purification Method:
Affinity purification
Gene ID:
5093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using hnRNP E1/PCBP1 antibody (CAB1044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
