Pyruvate carboxylase (PC) Rabbit Polyclonal Antibody (CAB6301)
- SKU:
- CAB6301
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
Product Name: | Pyruvate carboxylase (PC) Rabbit Polyclonal Antibody |
SKU: | CAB6301 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC)) (NP_001035806.1). |
Sequence: | HSMG LGSK FKEV KKAY VEAN QMLG DLIK VTPS SKIV GDLA QFMV QNGL SRAE AEAQ AEEL SFPR SVVE FLQG YIGV PHGG FPEP FRSK VLKD LPRV EGRP GASL PPLD LQAL EKEL VDRH GEEV TPED VLSA AMYP DVFA HFKD FTAT FGPL DSLN TRLF LQGP KIAE EFEV ELER GKTL HIKA LAVS DLNR AGQR QVFF ELNG QLRS ILVK DTQA MKEM HFHP KALK DVKG QIGA PMPG KVID IKVV AGAK VAKG QPLC VLSA MKME TVVT SPME GTVR KVHV TKDM TLEG DDLI LEIE |
Tested Applications: | WB IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | PCB; Pyruvate carboxylase (PC) |
Positive Sample: | HepG2,HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion matrix. |
Calculated MW: | 130kDa |
Observed MW: | 130kDa |
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 5091 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |