The PC Polyclonal Antibody (CAB6301) is a valuable tool for researchers studying immune regulation and cell surface molecules. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the CD300A protein, the antibody enables detection and analysis in various cell types, making it ideal for studies in immunology and cancer research.CD300A, also known as an immune inhibitory receptor, plays a critical role in immune homeostasis by regulating inflammation and inhibiting allergic reactions.
Its significance in immune response modulation makes it a valuable target for research into diseases like cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the activity of CD300A is crucial for developing therapies that harness or modulate immune function in these contexts. The PC Polyclonal Antibody (CAB6301) offers researchers a reliable tool for investigating the role of CD300A in these important areas of study.
Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC)) (NP_001035806.1).
WB,1:500 - 1:1000 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
PCB; Pyruvate carboxylase (PC)
Positive Sample:
HepG2,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion matrix.
Calculated MW:
130kDa
Observed MW:
130kDa
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5091
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Pyruvate carboxylase (PC) Rabbit pAb (CAB6301) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
