The Pax6 Rabbit Monoclonal Antibody (CAB19099) is a reliable tool for researchers studying the Pax6 protein, a key transcription factor involved in eye development and neurogenesis. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting Pax6 in human samples, making it a valuable asset for immunohistochemistry, immunofluorescence, and Western blotting applications.Pax6 is essential for proper eye development and function, as well as for the regulation of neural stem cell differentiation.
Dysregulation of Pax6 has been linked to various ocular disorders, making it an important target for studies related to visual system development and diseases. By using the Pax6 Rabbit Monoclonal Antibody, researchers can gain valuable insights into the expression patterns and functions of Pax6 in different tissues and cell types, advancing our understanding of its role in both normal and pathological conditions.
Product Name:
PAX6 Rabbit Monoclonal Antibody
SKU:
CAB19099
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 323-422 of human PAX6 (NP_000271.1).
This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain.
Purification Method:
Affinity purification
Gene ID:
5080
Clone Number:
ARC0367
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using PAX6 Rabbit mAb (CAB19099) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
