The Pax6 Polyclonal Antibody (CAB7334) is a powerful tool for researchers studying the role of Pax6, a key transcription factor essential for eye development and CNS regulation. This antibody, raised in rabbits, is highly specific and sensitive for detecting Pax6 protein in a variety of samples, particularly in human tissues. Validated for use in Western blot applications, it enables accurate detection and quantification of Pax6 levels in different cell types.Pax6, known as the master regulator of eye development, is crucial for the formation of the eye and brain during embryonic development. Dysfunction of this transcription factor has been linked to various eye disorders, such as aniridia and congenital cataracts, as well as neurodevelopmental conditions like autism spectrum disorders.
By studying Pax6 expression and activity, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic targets.Given its importance in developmental biology and neurology, the Pax6 Polyclonal Antibody is an invaluable tool for investigating the role of Pax6 in health and disease. Its high specificity and sensitivity make it an essential reagent for studies in ophthalmology, neuroscience, and developmental biology, offering researchers the ability to further explore the functions of Pax6 in various biological contexts.
Product Name:
PAX6 Rabbit Polyclonal Antibody
SKU:
CAB7334
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAX6 (NP_000271.1).
This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain.
Purification Method:
Affinity purification
Gene ID:
5080
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PAX6 Rabbit pAb (CAB7334) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
