The Pax5 Polyclonal Antibody (CAB3068) is a valuable tool for researchers studying the transcription factor Pax5, which plays a critical role in B-cell development and differentiation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Pax5, also known as B-cell-specific activator protein (BSAP), is essential for the development of B cells and the regulation of their function. Dysregulation of Pax5 has been implicated in various B-cell malignancies, making it a target of interest in cancer research.
By using the Pax5 Polyclonal Antibody, researchers can detect and analyze Pax5 protein levels in different cell types, providing valuable insights into the role of Pax5 in normal B-cell development and malignant transformation. This antibody is a valuable tool for studies in immunology, oncology, and developmental biology, helping to advance our understanding of B-cell development and the pathogenesis of B-cell-related diseases.
Product Name:
PAX5 Rabbit Polyclonal Antibody
SKU:
CAB3068
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 150-300 of human PAX5 (NP_057953.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ALL3; BSAP; PAX-5; PAX5
Positive Sample:
Daudi,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
42kDa
Observed MW:
45kDa
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5079
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PAX5 Rabbit pAb (CAB3068) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
