The Pax5 Polyclonal Antibody (CAB12048) is a valuable tool for researchers studying Pax5, a transcription factor known for its role in regulating B cell development and function. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By targeting the Pax5 protein, this antibody enables precise detection and analysis of Pax5 expression in various cell types, making it an essential component for studies in immunology and cancer research.
Pax5, also referred to as B-cell specific activator protein, plays a crucial role in B cell lineage commitment and differentiation, making it a key player in the immune response. Dysregulation of Pax5 has been implicated in various diseases, including leukemia and lymphoma, highlighting its potential as a therapeutic target. By understanding the function of Pax5, researchers can gain insights into the mechanisms underlying these diseases and develop targeted therapies to combat them effectively.
Product Name:
PAX5 Rabbit Polyclonal Antibody
SKU:
CAB12048
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAX5 (NP_057953.1).
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5079
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Daudi, using PAX5 Rabbit pAb (CAB12048) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
