The Pax3 Monoclonal Antibody is a crucial tool for research involving the Pax3 protein, a transcription factor known for its role in the development of muscle tissues, particularly in the formation of skeletal muscles. This highly specific antibody, produced using hybridoma technology, is reactive with human samples and is validated for use in various applications, including immunohistochemistry and flow cytometry.Pax3 is a key regulator of embryonic development, specifically in the formation of skeletal muscles and neural crest cells. Aberrant expression or function of Pax3 has been implicated in various muscular dystrophies and cancers, making it a valuable target for studies in developmental biology and oncology.
The Pax3 Monoclonal Antibody allows for the detection and analysis of Pax3 protein expression in cells and tissues, providing insights into its role in normal development and disease progression.Researchers studying muscle development, cancer biology, and regenerative medicine will find the Pax3 Monoclonal Antibody to be an essential tool in their investigations. Its specificity and reliability in detecting Pax3 protein make it an invaluable resource for understanding the molecular mechanisms underlying muscle formation and the potential therapeutic targets for muscle-related disorders.
Product Name:
PAX3 Monoclonal Antibody
SKU:
CAB22293
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAX3 (NP_852122.1).
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.
Purification Method:
Affinity purification
Gene ID:
5077
Clone Number:
ARC57259
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PAX3 antibody (CAB22293) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
