The PAWR Polyclonal Antibody (CAB3063) is a valuable tool for researchers studying the PAWR protein, also known as PRKC apoptosis WT1 regulator. PAWR is involved in apoptosis, cell cycle regulation, and tumor suppression, making it a key player in cancer research and other cellular processes.This antibody, raised in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for a variety of research applications. It has been validated for use in Western blotting, immunofluorescence, and immunohistochemistry, allowing for the detection and analysis of PAWR expression in different cell types and tissues.
The significance of PAWR in apoptosis regulation and tumor suppression highlights its potential as a therapeutic target for cancer treatment. By studying the activity and expression of PAWR, researchers can gain insights into the mechanisms underlying cancer development and progression, leading to the development of novel therapeutic strategies. The PAWR Polyclonal Antibody is a valuable tool for advancing research in oncology and related fields.
Product Name:
PAR4 Rabbit Polyclonal Antibody
SKU:
CAB3063
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PAR4 (NP_002574.2)
This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.
Purification Method:
Affinity purification
Gene ID:
5074
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PAR4 antibody (CAB3063) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
