The PARP4 Polyclonal Antibody (CAB8343) is a specialized research tool designed for studying PARP4, a protein involved in DNA repair and genome stability. This antibody is produced in rabbits and is highly specific to human samples, making it ideal for experiments focused on understanding the role of PARP4 in cellular processes.PARP4, also known as poly(ADP-ribose) polymerase 4, plays a key role in DNA damage response pathways, particularly in repairing single-strand breaks in DNA. Dysregulation of PARP4 has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions.
With its high reactivity and specificity, the PARP4 Polyclonal Antibody allows for accurate detection and analysis of PARP4 protein levels in a variety of cell types and experimental settings. Researchers in the fields of genetics, oncology, and neurobiology can benefit from using this antibody in techniques such as Western blotting to further unravel the functions of PARP4 in health and disease.
Product Name:
PARP4 Rabbit Polyclonal Antibody
SKU:
CAB8343
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP4 (NP_006428.2).
This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.
Purification Method:
Affinity purification
Gene ID:
143
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PARP4 antibody (CAB8343) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
