The PARP1 Polyclonal Antibody (CAB22534) is a valuable tool for research involving PARP1, a key enzyme involved in DNA repair and regulation of gene expression. This antibody, produced in rabbits, has high specificity and sensitivity for detecting PARP1 in human samples, making it ideal for use in Western blot applications. By binding to the PARP1 protein, the antibody allows for the detection and analysis of this important enzyme in various cell types.PARP1 is a critical player in cellular processes such as DNA damage repair, chromatin remodeling, and apoptosis. Dysregulation of PARP1 has been associated with a range of diseases, including cancer, neurodegenerative disorders, and chronic inflammation.
Studying the function and regulation of PARP1 is essential for understanding the molecular mechanisms underlying these conditions and for developing targeted therapies.Overall, the PARP1 Polyclonal Antibody (CAB22534) is a valuable tool for researchers studying the role of PARP1 in various biological processes and diseases. Its high specificity and sensitivity make it a reliable choice for Western blot analysis and other experimental techniques.
Product Name:
PARP1 Polyclonal Antibody
SKU:
CAB22534
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human PARP1. (NP_001609.2).
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Purification Method:
Affinity purification
Gene ID:
142
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Jurkat, using PARP1 antibody (CAB22534) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
_WB_01__83791.1701342911.jpg?c=2&imbypass=on "Western blot analysis of Jurkat, using PARP1 antibody (CAB22534) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
