The PARP1 Polyclonal Antibody (CAB0942) is a valuable tool for researchers studying Poly (ADP-ribose) polymerase 1 (PARP1), a critical enzyme involved in various cellular processes, including DNA repair, chromatin remodeling, and cell death. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blotting applications.PARP1 plays a significant role in DNA damage response and is a potential target for cancer therapy. By binding to PARP1, this antibody enables the detection and analysis of PARP1 expression levels in different cell types, facilitating research in fields such as oncology, genomics, and drug development.
Understanding the functions and regulatory mechanisms of PARP1 is crucial for elucidating its role in disease pathogenesis, particularly in cancer progression and therapeutic resistance. The PARP1 Polyclonal Antibody (CAB0942) serves as a reliable tool for investigating the complex biology of PARP1 and its implications for targeted treatment strategies in various disease conditions.
Product Name:
[KO Validated] PARP1 Rabbit Polyclonal Antibody
SKU:
CAB0942
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Purification Method:
Affinity purification
Gene ID:
142
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PARP1 Rabbit pAb (CAB0942) at 1:3000 dilution.HeLa cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
