The PARN Polyclonal Antibody (CAB4002) is an essential tool for researchers studying PARN, a key enzyme involved in RNA metabolism and RNA degradation processes. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications.PARN, a poly(A)-specific ribonuclease, plays a critical role in regulating gene expression by controlling the length of poly(A) tails on messenger RNA molecules.
Dysregulation of PARN has been implicated in various diseases, including cancer and neurological disorders.By targeting the PARN protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and RNA research. Understanding the function and activity of PARN is essential for unraveling its role in disease pathogenesis and developing targeted therapies.
Product Name:
PARN Rabbit Polyclonal Antibody
SKU:
CAB4002
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PARN (NP_002573.1).
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5073
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PARN antibody (CAB4002) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 150s.
