The PARL Polyclonal Antibody (CAB8232) is specifically designed for researchers studying the PARL protein, a key player in mitochondrial biology and cell survival mechanisms. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the PARL protein, this antibody allows for precise detection and analysis in various cell types, making it an essential tool for studies in mitochondrial dysfunction, cell death pathways, and neurodegenerative diseases.PARL, also known as presenilin-associated rhomboid-like protein, is a crucial regulator of mitochondrial function, including mitochondrial dynamics, apoptosis, and cellular stress responses.
Its pivotal role in maintaining cellular homeostasis makes it an attractive target for research into diseases such as Alzheimer's, Parkinson's, and metabolic disorders. By understanding the function of PARL, researchers can uncover potential therapeutic targets for treating these debilitating conditions and advancing our knowledge of mitochondrial biology.
Product Name:
PARL Rabbit Polyclonal Antibody
SKU:
CAB8232
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PARL (NP_001032728.1).
This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease.
Purification Method:
Affinity purification
Gene ID:
55486
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PARL antibody (CAB8232) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
