The PAPSS1 Polyclonal Antibody (CAB13773) is a valuable tool for research involving PAPSS1, an enzyme involved in the biosynthesis of sulfated molecules like heparan sulfate and cholesterol sulfate. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.PAPSS1 plays a crucial role in sulfation reactions, which are vital for the structure and function of various molecules in the body. Dysregulation of PAPSS1 activity has been implicated in diseases such as cancer, cardiovascular disorders, and metabolic syndromes.
Therefore, studying the expression and localization of PAPSS1 using this antibody can provide valuable insights into the mechanisms underlying these conditions.By enabling the detection and analysis of PAPSS1 in different cell types and tissues, the CAB13773 antibody is an essential tool for researchers in biochemistry, molecular biology, and pharmaceutical sciences. Its high specificity and sensitivity make it an ideal choice for investigating the role of PAPSS1 in normal physiological processes and disease pathology.
Product Name:
PAPSS1 Rabbit Polyclonal Antibody
SKU:
CAB13773
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 190-260 of human PAPSS1 (NP_005434.4).
Sequence:
SEYE KPEA PELV LKTD SCDV NDCV QQVV ELLQ ERDI VPVD ASYE VKEL YVPE NKLH LAKT DAET LPAL KIN
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
SK1; PAPSS; ATPSK1; PAPSS1
Positive Sample:
LO2,HT-29,293T,Mouse brain,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
cytosol
Calculated MW:
71kDa
Observed MW:
71kDa
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).
Purification Method:
Affinity purification
Gene ID:
9061
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PAPSS1 Rabbit pAb (CAB13773) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.