The PAPPA Antibody (PACO20169) is a valuable tool for researchers studying the pregnancy-associated plasma protein A (PAPPA), a metalloproteinase involved in the regulation of insulin-like growth factor (IGF) bioavailability. This polyclonal antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.PAPPA plays a crucial role in the cleavage of IGF-binding proteins, leading to the release of bioactive IGF. Dysregulation of PAPPA expression has been linked to various diseases, including cancer and cardiovascular disorders. By targeting PAPPA with this antibody, researchers can investigate its role in these pathological conditions and explore its potential as a therapeutic target.
This antibody enables the detection and analysis of PAPPA in different cell types, providing valuable insights into its function and regulation. Its versatility makes it an essential tool for studies in reproductive biology, cancer research, and metabolic disorders. By understanding the mechanisms underlying PAPPA activity, researchers can ultimately develop novel strategies for treating diseases associated with dysregulated IGF signaling pathways.
Antibody Name:
PAPPA Antibody (PACO20169)
Antibody SKU:
PACO20169
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human PAPPA
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20169(PAPPA Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO20169(PAPPA Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses.
Synonyms:
pregnancy-associated plasma protein A, pappalysin 1
UniProt Protein Function:
PAPPA: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Belongs to the peptidase M43B family.
UniProt Protein Details:
Protein type:EC 3.4.24.79; Secreted; Secreted, signal peptide; Protease
Chromosomal Location of Human Ortholog: 9q33.2
Cellular Component: membrane; extracellular region
Molecular Function:metallopeptidase activity; zinc ion binding; metalloendopeptidase activity
This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017]
