The PAPD4 Polyclonal Antibody (CAB13238) is a valuable tool for researchers studying PAPD4, an enzyme involved in RNA metabolism and mitochondrial protein synthesis. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the PAPD4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal resource for investigations in molecular biology and mitochondrial research.
PAPD4, also known as poly(A) polymerase delta 4, plays a crucial role in post-transcriptional RNA modification and regulation, contributing to the synthesis and maintenance of mitochondrial proteins. Its involvement in RNA metabolism makes it a prominent target for studies on mitochondrial function, RNA processing, and gene expression. Understanding the function and regulation of PAPD4 is essential for unraveling its contribution to cellular processes and its potential implications in various diseases and conditions.
Product Name:
PAPD4 Rabbit Polyclonal Antibody
SKU:
CAB13238
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAPD4 (NP_776158.2).
Enables 5'-3' RNA polymerase activity and polynucleotide adenylyltransferase activity. Involved in RNA metabolic process and negative regulation of RNA catabolic process. Predicted to be located in nucleus. Predicted to be part of nuclear RNA-directed RNA polymerase complex. Predicted to be active in cytosol.
Purification Method:
Affinity purification
Gene ID:
167153
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PAPD4 Rabbit pAb (CAB13238) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
