The Pan-Trimethyl Lysine Polyclonal Antibody (CAB18292) is a valuable tool for researchers studying protein methylation, a post-translational modification that plays a crucial role in regulating protein function. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards trimethylated lysine residues in a wide range of protein samples, making it an ideal choice for Western blot and immunohistochemistry applications.Trimethylation of lysine residues is a key mechanism for regulating various cellular processes, including gene expression, signal transduction, and protein-protein interactions. Dysregulation of lysine methylation has been linked to various diseases, including cancer, neurological disorders, and metabolic conditions.
By using the Pan-Trimethyl Lysine Polyclonal Antibody, researchers can investigate the impact of lysine methylation on protein function and disease pathology, leading to potential therapeutic targets and biomarkers for diagnostic purposes.In conclusion, the Pan-Trimethyl Lysine Polyclonal Antibody is a reliable tool for investigating the role of protein methylation in cellular processes and disease development. Its high specificity and sensitivity make it a valuable asset for researchers in the fields of molecular biology, biochemistry, and biomedicine. By utilizing this antibody, researchers can deepen their understanding of protein methylation dynamics and its implications for human health.
Product Name:
pan-TriMethyl-lysine Rabbit Polyclonal Antibody
SKU:
CAB18292
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing trimethylated K.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
C6,Mouse liver,Rat liver,HeLa,MCF7
Observed MW:
18-55kDa
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).The post-translational ε-amino lysine methylated proteins is an important reversible modification which plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription. Methylation of lysine residues is modulated by specific counteractive enzymes including lysine methylases (KMTs) and demethylases (KDMs). Lysine trimethylation occurs in both histones and non-histone substratres. It has become promising targets for discovery of anti-cancer drugs.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using pan-TriMethyl-lysine pAb (CAB18292) at 1:500 dilution.HeLa and MCF7 cells were treated by ADOX (100 μM) for 24 hours .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
