The Pan-PKC Polyclonal Antibody (CAB17922) is specifically designed for research involving Protein Kinase C (PKC), a family of enzymes that play a crucial role in cell signaling and regulation. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in a variety of applications, including Western blotting. Protein Kinase C (PKC) is involved in various cellular processes, including cell proliferation, differentiation, and apoptosis, making it a key player in cancer and other disease states.
By targeting PKC, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. The Pan-PKC Polyclonal Antibody facilitates the detection and analysis of PKC in different cell types, making it a versatile tool for studies in cell biology, molecular biology, and cancer research. Its high specificity and sensitivity make it an ideal choice for researchers looking to further their understanding of PKC and its role in human health and disease.
Product Name:
pan-PKC Rabbit Polyclonal Antibody
SKU:
CAB17922
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 572-672 of humanPRKCA (NP_002728.2)
cytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, mitochondrial membrane, mitochondrion, nucleoplasm, perinuclear region of cytoplasm, plasma membrane
Calculated MW:
59kDa/67kDa/74- 83kDa
Observed MW:
83kDa
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption.
Purification Method:
Affinity purification
Gene ID:
5578/5579/5580/5581/5583/5588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using pan-PKC Rabbit pAb (CAB17922) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
