The Pan Phospho-T Polyclonal Antibody is a valuable tool for researchers studying signaling pathways involving phosphorylation events. This antibody, raised in rabbits, is highly specific and reactive with phosphorylated threonine residues in proteins across various species, making it ideal for use in Western blot and immunohistochemistry applications.Phosphorylation of threonine residues is a key post-translational modification that regulates many cellular processes, including cell growth, proliferation, and differentiation. By targeting phosphorylated threonine residues in proteins, researchers can gain insights into the activation status of signaling pathways and the role of phosphorylation in disease processes.
The Pan Phospho-T Polyclonal Antibody allows for the detection and analysis of phosphorylated threonine residues in a wide range of proteins, providing important information for studies in cell signaling, cancer biology, and other fields. Its high specificity and sensitivity make it a valuable tool for researchers seeking to unravel the complex mechanisms of phosphorylation-mediated cellular regulation.
Product Name:
Pan Phospho-T Polyclonal Antibody
SKU:
CABP1513
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
A-431,NIH/3T3
Conjugate:
Unconjugated
Observed MW:
10-250kDa/>10kDa
nan
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit pAb (CABP1513) at 1:900 dilution. A-431 cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
