Pan Mono-Methyl lysine Rabbit Polyclonal Antibody (CAB18293)
The Pan Mono-Methyl Lysine Polyclonal Antibody (CAB18293) is a valuable tool for researchers studying lysine mono-methylation, a post-translational modification of histone proteins that plays a crucial role in gene regulation. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting mono-methylated lysine residues in various protein samples, with a particular affinity for human samples.The antibody is validated for use in a variety of applications, including Western blotting, immunoprecipitation, and immunofluorescence, allowing for the detection and analysis of mono-methylated lysine proteins in different cell types and tissues. Its versatility makes it ideal for studies in epigenetics, chromatin remodeling, and gene expression regulation.
Mono-methylation of lysine residues has been associated with various biological processes, including transcriptional activation, DNA repair, and cell differentiation. Dysregulation of lysine mono-methylation has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic conditions. By using the Pan Mono-Methyl Lysine Polyclonal Antibody, researchers can gain valuable insights into the role of mono-methylated lysine residues in health and disease, paving the way for the development of novel therapeutic strategies targeting epigenetic modifications.
Product Name:
Pan Mono-Methyl lysine Rabbit Polyclonal Antibody
SKU:
CAB18293
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing monomethylated K.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
HeLa,MCF7,NIH/3T3,C6,Mouse liver,Rat liver
Observed MW:
17-90kDa
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb (CAB18293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
