The Pan-Methyl Histidine Polyclonal Antibody (CAB17948) is a valuable tool for researchers studying histidine methylation in proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By specifically binding to pan-methyl histidine residues, this antibody allows for the detection and analysis of proteins modified by this post-translational modification.Histidine methylation is a crucial regulatory mechanism in various cellular processes, including signal transduction, protein-protein interactions, and gene expression.
Dysregulation of histidine methylation has been implicated in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. Understanding the role of pan-methyl histidine in these diseases may provide valuable insights for developing targeted therapies.Researchers in the fields of biochemistry, cell biology, and molecular biology can benefit from the use of the Pan-Methyl Histidine Polyclonal Antibody for their experimental studies. Its specificity and sensitivity make it an essential tool for investigating the role of histidine methylation in health and disease.
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using pan-methyl-Histidine antibody (CAB17948) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 300s.
