Pan DiMethyl-lysine Rabbit Polyclonal Antibody (CAB18296)
The Pan-Dimethyl Lysine Polyclonal Antibody (CAB18296) is a versatile tool for researchers studying protein methylation, a key epigenetic modification implicated in gene regulation and various cellular processes. This antibody, derived from rabbit, exhibits high specificity and sensitivity for detecting dimethyl lysine residues across a range of protein samples in Western blot and immunohistochemistry applications.Dimethyl lysine modifications play crucial roles in diverse biological processes, including gene expression, DNA repair, and signal transduction pathways. The Pan-Dimethyl Lysine Polyclonal Antibody targets this modification, allowing for precise identification and quantification of methylated lysine residues on proteins in different cell types and tissues.
This makes it an essential tool for researchers investigating epigenetic regulation, chromatin dynamics, and the role of protein methylation in health and disease.By providing a reliable means of detecting dimethyl lysine modifications, the Pan-Dimethyl Lysine Polyclonal Antibody enables researchers to explore the functional implications of protein methylation in various biological contexts. Its ability to specifically recognize dimethyl lysine residues offers valuable insights into the regulation of cellular processes and pathways associated with epigenetic modifications, contributing to our understanding of complex biological mechanisms and potential therapeutic strategies targeting protein methylation.
Product Name:
Pan DiMethyl-lysine Rabbit Polyclonal Antibody
SKU:
CAB18296
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing dimethylated K.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
NIH/3T3,C6,Mouse liver,Rat liver,HeLa,MCF7
Observed MW:
17-48kDa
The lysine methylation chemical reaction is the reversible addition of one, two or three methyl groups to the ε-nitrogen of a lysine side chain, forming monomethylated, dimethylated and trimethylated derivatives (referred to here as ‘Kme1’, ‘Kme2’ and ‘Kme3’).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan DiMethyl-lysine Rabbit pAb (CAB18296) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
