The Pan-AKT Polyclonal Antibody (CAB18120) is a valuable tool for researchers studying the AKT signaling pathway, a key regulator of cell growth, proliferation, survival, and metabolism. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications to detect and analyze AKT protein levels.AKT, also known as protein kinase B, plays a critical role in various cellular processes, including cell cycle progression, apoptosis, and metabolism. Dysregulation of the AKT pathway has been implicated in various diseases, including cancer, diabetes, and neurological disorders.
Therefore, understanding the activity of AKT and its downstream targets is essential for developing targeted therapies for these conditions.By using the Pan-AKT Polyclonal Antibody, researchers can gain valuable insights into the activation status of the AKT pathway in different cell types and tissues. This antibody enables precise detection and quantification of AKT protein levels, facilitating further investigation into the role of AKT in disease progression and potential therapeutic interventions.
Product Name:
Pan-AKT Rabbit Polyclonal Antibody
SKU:
CAB18120
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 311-480 of human AKT1/AKT2/AKT3 (NP_005154.2).
Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.
Purification Method:
Affinity purification
Gene ID:
207/208/10000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Pan-Akt Rabbit pAb (CAB18120) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
