The PAM16 Polyclonal Antibody (CAB9875) is a valuable tool for researchers studying PAM16, a protein involved in mitochondrial protein import and assembly. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the PAM16 protein, this antibody enables the detection and analysis of PAM16 in various cell types, making it an ideal choice for research in the fields of biochemistry and cell biology.PAM16 is a key player in the process of importing mitochondrial proteins and ensuring their proper assembly, making it essential for mitochondrial function and overall cell health.
Research on PAM16 is crucial for understanding mitochondrial biology and its implications for diseases related to mitochondrial dysfunction, such as neurodegenerative disorders and metabolic diseases. By studying PAM16 and its role in mitochondrial protein import, researchers can uncover new insights into cellular processes and potentially develop targeted therapies for mitochondrial disorders.
Product Name:
PAM16 Rabbit Polyclonal Antibody
SKU:
CAB9875
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-125 of human PAM16 (NP_057153.8).
This gene encodes a mitochondrial protein involved in granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling. This protein also plays a role in the import of nuclear-encoded mitochondrial proteins into the mitochondrial matrix and may be important in reactive oxygen species (ROS) homeostasis. Mutations in this gene cause Megarbane-Dagher-Melike type spondylometaphyseal dysplasia, an early lethal skeletal dysplasia characterized by short stature, developmental delay and other skeletal abnormalities.
Purification Method:
Affinity purification
Gene ID:
51025
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PAM16 antibody (CAB9875) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
