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PAK2 (Phospho-Ser20) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB01352
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Death
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
€599
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Description

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PAK2 (Phospho-Ser20)Colorimetric Cell-Based ELISA Kit

The Human PAK2 Phospho-Ser20 Colorimetric Cell-Based ELISA Kit is specifically designed for the accurate detection of phosphorylated PAK2 at serine 20 in cell lysates and tissue samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying intracellular signaling pathways.PAK2 is a key protein kinase that plays a crucial role in cell survival, proliferation, and migration. Phosphorylation at serine 20 is known to regulate PAK2 activity and its downstream signaling cascades, making it a valuable biomarker for understanding cell signaling mechanisms and potential therapeutic targets in diseases such as cancer and immune disorders.

With easy-to-follow protocols and quick assay procedures, the PAK2 Phospho-Ser20 Colorimetric Cell-Based ELISA Kit is an essential tool for researchers looking to study PAK2 phosphorylation dynamics and its functional implications. Get accurate and reliable results with this cutting-edge kit from Assay Genie.

Product Name:PAK2 (Phospho-Ser20) Colorimetric Cell-Based ELISA
Product Code:CBCAB01352
ELISA Type:Cell-Based
Target:PAK2 (Phospho-Ser20)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The PAK2 (Phospho-Ser20) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PAK2 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated PAK2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PAK2 phosphorylation.

Qualitative determination of PAK2 (Phospho-Ser20) concentration is achieved by an indirect ELISA format. In essence, PAK2 (Phospho-Ser20) is captured by PAK2 (Phospho-Ser20)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 5062, UniProt ID: Q13177, OMIM: 605022, Unigene: Hs.518530
Gene Symbol:PAK2
Sub Type:Phospho
UniProt Protein Function:PAK2: a protein kinase of the STE20 family. Critical effector linking RhoGTPases to cytoskeleton reorganization and nuclear signaling. Activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, STE; Kinase, protein; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasm; cytosol; nucleoplasm; perinuclear region of cytoplasm; plasma membrane

Molecular Function:ATP binding; identical protein binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; protein tyrosine kinase activator activity; Rac GTPase binding

Biological Process: actin cytoskeleton organization and biogenesis; apoptosis; axon guidance; cell migration; cell structure disassembly during apoptosis; innate immune response; negative regulation of apoptosis; negative regulation of protein kinase activity; peptidyl-serine phosphorylation; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; programmed cell death; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of defense response to virus by virus; regulation of growth; regulation of MAPKKK cascade; regulation of mitotic cell cycle; Rho protein signal transduction; signal transduction; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated protein kinase signaling pathway; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction

NCBI Summary:The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]
UniProt Code:Q13177
NCBI GenInfo Identifier:143811432
NCBI Gene ID:5062
NCBI Accession:Q13177.3
UniProt Secondary Accession:Q13177,Q13154, Q6ISC3,
UniProt Related Accession:Q13177
Molecular Weight:
NCBI Full Name:Serine/threonine-protein kinase PAK 2
NCBI Synonym Full Names:p21 protein (Cdc42/Rac)-activated kinase 2
NCBI Official Symbol:PAK2  
NCBI Official Synonym Symbols:PAK65; PAKgamma  
NCBI Protein Information:serine/threonine-protein kinase PAK 2
UniProt Protein Name:Serine/threonine-protein kinase PAK 2
UniProt Synonym Protein Names:Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:PAK2  
UniProt Entry Name:PAK2_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-PAK2 (Phospho-Ser20) Antibody, Anti-PAK2 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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